After staining the slide, the examination begins. Place the Blood drop in the center of the glass slide. The Thick blood smears are prepared for detecting the Blood parasites such as Plasmodium spp. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Too slow a slide push will accentuate poor leukocyte distribution, larger cells are pushed at the end of the slide. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white blood cells and specifies the morphologies of each leukocyte. Tnx for all keep it up am lab technician from Ethiopia. Clean slides with 70 to 90% alcohol and allow to dry. Now, Place a smooth, clean edge of the spreader slide on the specimen slide at an angle of about 30° – 45°. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells. The undiluted stain fixes and partially stains the smear. (adsbygoogle = window.adsbygoogle || []).push({}); Commonly, two types of blood smears are prepared for the diagnosis as well as prognosis of any abnormalities (if present). Wear gloves. Peripheral Blood Smear Preparation. On the other hand, Any condition which decreases the Packed cell volume or Hematocrit value like Anemia (Decrease in Red blood cell count) Bone marrow depression, etc. But, the slide should be washed with soap and water after touching its edge, to remove grease particles from its edges. The angle of the spreader varies with the thickness or Viscosity of the blood to obtain an ideal smear. Proper preparation of the blood smear is critical for correct interpretation and obtaining accurate results. Other names: peripheral smear, peripheral blood film, smear, blood film, manual differential, differential slide, blood cell morphology, blood smear analysis preparation of peripheral blood smear ⇒ Now, cover the well dried, thin blood smear with undiluted Leishman Stain solution by counting the drops of Leishman stain. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Precautions: Ensure that the whole drop of blood is picked up and spread. Maintain an even gentle pressure on the slide. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type, and characteristics of all the blood cells which aids the physician in diagnosing viral infections, bacterial infections, Parasitic infection and certain blood disorders such as Anemia, leukemia, bacterial or parasitic infection etc. This chapter describes techniques of preparation and interpretation of canine and feline blood smears and addresses integrating findings from blood smears with other parameters in CBCs. Such an area for examination is illustrated in the image below. Samples with heparin are unusable for preparation of the smears. Thin smears. The peripheral blood film (PBF) is a laboratory work-up that involves cytology of peripheral blood cells smeared on a slide. The blood smear in Figure 3C results when a slight amount of the blood droplet is … Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. Label pre-cleaned slides (preferably frosted-end) with patient’s name (or other identifier), date and time of collection. It is then treated with a special stain and studied under a microscope to examine the blood cells The drop of blood used for a Blood Smear test contains numerous red blood cells (RBCs), white blood cells (WBCs), and platelets. Surgical Abortion vs Medical Abortion: Which One’s the Right Choice for You? At one time, a blood smear was prepared on nearly everyone who had a CBC. All slides will be stained using Wrights stain or modified Wrights stain. Thin smears consist of blood spread in a layer such that the thickness decreases progressively toward the feathered edge. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. The Thin blood smear is prepared for studying the morphology of the blood cells and for the identification of microbial agents. Check out How to collect the blood sample by Venipuncture? A phlebotomist, a person specifically trained to draw blood, first cleans and sterilizes the injection site with an antiseptic. The drop should be in the center line of the glass slide. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. As basic as it is, PBF is invaluable in the characterization of various clinical diseases. ⇒ Using a lead pencil or glass marking pencil, write the Name, Identification no. BLOOD COLLECTION BY VENIPUNCTURE – SYRINGE AND VACUTAINER METHOD. A well-prepared blood smear is important to produce good results on analysis after doing a Giemsa stain, in identifying blood cells or/and demonstrating the presence of parasites in a sample. A blood smear is often used as a follow-up test to abnormal results on a complete blood count (CBC) to evaluate the different types of blood cells. At one time, a blood smear was prepared on nearly everyone who had a CBC. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. White Blood Cell Differential Case Simulator, White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions, Learn more about White Cell and Platelet Disorders: Peripheral Blood Clues to Nonneoplastic Conditions (online CE course). Many blood tests use computers to analyze results. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. 3. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. (Babesiosis) etc. Do not blow air on the slide from any source in an effort to enhance drying, it may distort the smear. For both methods, blood samples should be fresh and well-mixed at smear preparation time, and smears should be completely air-dried before staining. A Peripheral Blood Smear Examination is a procedure, which involves spreading a drop of blood thinly onto a glass slide. My greatest hobby is to teach and motivate other peoples to do whatever they wanna do in life. Blood smears are made by placing a well mixed drop of blood 1 to 2 mm in diameter 1/4 inch from the frosted edge of the slide. The blood smear is then fixed and stained with Romanowski stain for microscopic examination. Cannabidiol or CBD for Anxiety – Can Hemp Help You Calm Down? These margins are left onto the glass slide to get a region where the cells are spaced far enough apart to be counted and differentiated. E.D.T.A. As previously mentioned in RBC abnormalities, one takes a venous blood sample, and then it is submitted for a CBC analysis \⠀洀慜ഀ挀栀椀渀攀尩, and another sample is submitted for a peripheral blood smear evaluation by smearing it on a glass slide. Malaria Journal 2013; 12: 231. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. A reproducible blood smear review requires every peripheral smear be prepared for consistent cellular distribution and proper clarity. The conditions like Polycythemia (increase in the Red blood cell count), Dehydration or any condition which increases the Packed cell volume or Hematocrit value is associated with the increase in the viscosity of blood. 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