Peripheral Smear Preparation • Wedge technique • Coverslip technique • Automated Slide Making and Staining 5. The DLC involves the counting of 100 white blood cells (WBCs) noting the number, type… The final preparation is thin and uniform. of collection) Preparation of Blood Film: The slide should be clean. Left smear is unstained, right smear is stained with Wright-Giemsa stain. Blood smear 1. Blood smears – common errors The blood droplet on the left is too big and will result in a thick smear. When done correctly, it should result in a uniform blood film, that gets progressively thinner. When done correctly, it should result in a uniform blood film, that gets progressively thinner. Blood Smear Technique. A thick blood smear is made by spreading a large blood drop in a small area of about 1 cm which provides a better opportunity to detect various parasitic forms against a more transparent background. Proper preparation of the blood smear is critical for obtaining accurate results on the differential. The blood smear is dried, fixed to the slide with methanol, and stained to distinguish the various cell types. ICSH has published a “reference” staining method for blood films based on purified azure B and eosin Y solutions (ICHS, 2010). A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. Optical microscopy offers the ability to gather information and aid in continued research. Importantly, viewing blood smears under the microscope needs to be done shortly after blood collection employing sterile technique (**wearing gloves) from a disinfected site (wiping off 1st drop of blood). ** Be sure to take the utmost precaution and care when performing a microscope experiment. 4. blood cell (RBCs), background an d plasma of peripheral blood smear im age by using image processing and signal processing techniques. Selecting a mild technique for some or shape of b to the stains react or crystal violet is the cell. Online Microbiology and Biology Study Notes, Home » Parasitology » Thick Blood Smear and Thin Blood Smear, Last Updated on February 3, 2020 by Sagar Aryal. Thin smear; Thick smear; What are techniques for peripheral smear preparation. 3) Place the end of the spreader slide on the sample slide so that the short sided edge of the spreader is below the drop of blood. The Clinical Pathology Laboratory uses the wedge technique for preparation of blood smears. 2. The features of the application of the image processing technique for the analysis of a blood smear are highlighted. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. BLOOD SMEARS • Peripheral blood smear is a very important tool in the hematology lab • It provides rapid, reliable access to information about a variety of hematologic disorders • Examination of the peripheral blood smear is an inexpensive but powerful diagnostic tool in both children and adults • The smear offers a window into … Made with ♡ by Sagar Aryal. The Thin Blood smear is prepared by making a drop of well-mixed venous blood, 2mm in diameter at the center of a sterilized microscopic glass slide. blood drop 1cm from the end of the slide. Rapidly air-dry the smears. A second glass slide is used as a spreader, streaking the blood into a thin film across the glass slide. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. Samples with heparin are unusable for preparation of the smears. The test is essential if you need a blood transfusion or are planning to donate blood. The other is pusher or the spreader slide Drop of blood (2-3 mm) in diameter: The blood can be obtained from finger Heal Microhematochrit tubes (non heparinised for EDTA or Heparinised for capillary blood) Wooden stick or glass capillary pipette In live blood analysis, much the same questions and factors can be considered. b. White blood cells comprise Granulocytes which include Neutrophils, Eosinophils and Basophils and Agranulocytes which include Lymphocytes and Monocytes. For a blood smear test, a laboratory professional examines the slide under a microscope and looks at the size, shape, and number of different types of blood cells. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. Below is a step-by-step guide for veterinarians produced by the Department of Agriculture and Food, Western Australia for performing blood smears for diagnostic use. Blood films are examined in the investigation of hematological (blood) disorders and are routinely employed to look for blood parasites, such as those of malaria and filariasis Smear is finger shaped, very slightly rounded at the feathery edge: widest area of examination. Thick blood smears for blood parasites . The technique offers more control of the spreader slide. The following points highlight the top five types of Staining. There are many reasons why your doctor may order a blood smear. This technique requires at least two 3 × 1-inch (75 × 25-mm) clean glass slides. Entire whole drop of blood is picked up and spread Aseptic Technique and It’s Important in Microbiology, 24–28. E.D.T.A. What are these specifications? Nickel method Blood smear technique. One slide serves as the blood smear slide and the other as the spreader slide. 3) Blood sample 4) Microscope. After one minute staining with black Quink®, the walls of the red blood cells (erythrocytes) Blood smear technique When staining either Romanowsky, Wright's or Giemsa stain are used or a combination thereof, usually simply Wright's stain. Internal reflections or contamination in the sample and/or in the optics can cause refractiles. Perhaps there is a problem with the optics and magnifications. Right – blood as seen at 400x under light microscope. , the goal is to not perform diagnoses but to, technique and processes needed to view a blood smear under. After heat-fixing, the fixed smear can be stained on a staining rack using different types of dyes depending on the staining technique used. The wedge smear. Blood smears are needed for microscopic examination of the blood. A small drop of blood is placed on the midline at the end of a glass slide. Precautions! These include: Red blood cells have a characteristic pink appearance due to their high content of hemoglobin. Examination of the peripheral blood smear should be considered, along with review of the results of peripheral blood counts and red blood cell indices, an essential component of the initial evaluation of all patients with hematologic disorders. Learn how your comment data is processed. The blood droplet on the right is the preferred size. Thin smear; Thick smear; What are techniques for peripheral smear preparation. Line concentration technique. a. A blood film—or peripheral blood smear—is a thin layer of blood smeared on a glass microscope slide and then stained in such a way as to allow the various blood cells to be examined microscopically. Peripheral blood smear examinaon in addion with complete blood count (CBC) by the automated hematology analyzers can provide a more accurate report on categorizing different types of anemia. A single smear can be made per slide (smear running the length of the slide) or two (or even three) smears can share a slide, with the smears running the width of the slide. Increasing in progression, it is best to view smears up to a total magnification of 1000x using the 10x ocular. The blood elements (including parasites, if any) are more concentrated (app. can be trapped water in an inactive cell membrane. Smears should be made within 1 hour of blood collection from EDTA specimens stored at room temperature to avoid distortion of cell morphology Blood smears can also be made … Some additional guidelines should be followed to create the best blood smear… A Peripheral blood smear (PBS) or Blood film is required to be made from capillary blood or from a drop of blood from an EDTA (anticoagulated) blood sample. The MicroscopeMaster website is for educational purposes only. Simple Staining 2. Place a small drop of blood, or one side about 1-2 cm from one end. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa staining technique, Importance, and applications of blood smears, in detail. Firstly, what stain. Neutrophils are displayed as a deep blue/purple nucleus under the microscope. The present study was aimed at modifying the centrifuged blood smear (modified centrifuged blood smear or MCBS), to make it a feasible and standardized procedure. Blood smears are mostly done for Differential Leukocyte count (DLC)i.e it quantifies the white … Jeffrey C. Pommerville (2017), Fundamentals of Microbiology, 11th Edition, Jones & Bartlett Learning, LCC , Ascend Learning Company 8. This preparation is allowed to dry and then fixed with an appropriate Romanowski stain, depending on your objective. Images are used with permission as required. Some additional guidelines should be followed to create the best blood smear. Quantitative importance enables the numbering of blood cells while the qualitative function is to demonstrate and identify the cell morphologies, including types of leukocytes, erythrocytes, monocytes, and platelets. Increasing in progression, it is best to view smears up to a total magnification of 1000x using the 10x ocular. 2. This is done in a smooth and quick motion. Scientific understanding changes over time. thick smear ma de-staining nagarda pani hunchha sir ??? The Procedure of Giemsa staining varies as per the purpose of staining that means whether the staining is done for the examination of Blood cells or to find the Parasites in the blood smear and accordingly the Blood smears are prepared as Thin Blood films or Thick blood films. Thicker areas dry from outside to inside. Below, we discuss the procedures for preparing both thin and thick smear for Giemsa stainingtechnique, Importance, and applications of blood smears, in detail. An experienced observer should be comfortable in doing this so as to deliver results in a lab accurately and quickly. Steps of microbial smear preparation : Types of Stains 7. artifact or a stain related artifact which are, usually more evident during humid summer months. NOTE: Dry smears are the best for staining, so ensure your smear is completely dry before applying a staining technique. Therefore HPV co-testing is advised. 30×) than in an equal area of a thin smear. © 2021 Microbe Notes. Name the types of peripheral blood smear. A blood smear is a sample of blood that's tested on a specially treated slide. A new piece of optical equipment/dye may alter your signal-image. WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. It may be used to help diagnose and/or monitor numerous conditions that affect blood cell populations. Make the blood smear without delay. Materials 2 (75mm x 25mm) clean glass slides. Using a high quality clean glass slide (flat, no distortions and corrosion resistant) of 75mm X 25mm and 1mm thickness is ideal. It can be performed with very small volumes of blood. Most commonly blood smears are used for the Differential Leukocyte count (DLC). Endothelial progenitor cells (EPCs) are progenitors with the ability to produce functional endothelial cells. blood cell (RBCs), background an d plasma of peripheral blood smear im age by using image processing and signal processing techniques. Here is a great microscope to view live blood: OMAX 40X-2000X USB3 14MP PLAN Trinocular Darkfield Super Bright LED Lab Microscope, Return from Blood Smear to Microscope Slide Preparation, Privacy Policy by Hayley Anderson at MicroscopeMaster.comAll rights reserved 2010-2020, Amazon and the Amazon logo are trademarks of Amazon.com, Inc. or its affiliates. preparation like poor spreading technique. A small drop of blood is placed on the midline at the end of a glass slide. ing and then drying before staining. There are more than 100 types of HPV, of which at least 13 are cancer-causing (also known as high-risk type). Using a high quality clean glass slide (flat, no distortions and corrosion resistant) of 75mm X 25mm and 1mm thickness is ideal. Sexually acquired infection with certain types of HPV (Human papillomavirus) is a major cause of cervical cancer. When held up in light: feathery edge should show rainbow appearance. This is critical for optimal results. The basic one-step smearing technique (Abele et al, 1985) is designed to process a harvest consisting of one or two droplets of semisolid tissue material. Some of these include:1 1. to further evaluate abnormalities found on a complete blood count (CBC) such as a high or low red blood cell count, white blood cell count, or platele… Place a small drop of deposit near one end of glass slide. Moreover, the paent doesn't Peripheral blood film or Peripheral blood smear or Peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological (blood) disorders. Blood smear analysis is a well known technique in medical laboratories. Blood films are typically stained by Romanowsky dyes (consisting of a variety of thiazines and eosins) (Henry, 1998). Blood smear analysis allows quantitation of the different types of leukocytes (called the differential count), estimation of the platelet count, and detection of morphologic abnormalities that may be indicators of pathophysiologic processes. Using light microscopy, imaging is done with a 10x ocular and 10x objective at first. The most common technique of blood smear preparation is called the “wedge or push” technique. Collect blood in an EDTA tube and make the smears when back at the clinic. As a general rule for judging the quality of a stained blood film, the laboratory must ensure that all cell types in a blood film can be identified reliably by the staining procedure. The Thin Peripheral Blood smear is made by placing a well-mixed drop of blood 1 to 2 mm in diameter & 1/4 inch from the edge of the clean microscopic glass slide. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. this allows them to be used for the detection of blood parasites in the blood samples. The examination of blood films stained with Wright's st … for the hobbyist who enjoys furthering their knowledge or for the student needing some clarification. For turbid samples the blood smear technique is used (see preparing a blood film), or can be modified with the line concentration technique. Below, we discuss the procedures for preparing both thin and thick smear for, Collect blood sample by venipuncture and put in a clean test tube. They appear as biconcave discs of uniform shape and size (7.2 microns) that lack organelles and granules. Left - unstained blood smear on slide. A blood smear is a sample of blood that's tested on a specially treated slide. The drop should be … Endospore Staining. The MicroscopeMaster website is for educational purposes only. Allow the slide to air dry and stain with an appropriate staining technique. Rapid air drying of smear to preserve cell morphologies, Regular use of the technique to produce useful blood smears, https://paramedicsworld.com/hematology-stainings/giemsa-staining-technique-principle-preparation-procedure-interpretation/medical-paramedical-studynotes, https://paramedicsworld.com/hematology-practicals/preparation-peripheral-blood-smear/medical-paramedical-studynotes, https://www.vetstream.com/treat/felis/technique/blood-smear, Papanicolaou Staining (Pap stain) for Pap Smear / Pap Test, Blood Cells- Definition and Types with Structure and Functions, Staphylococcus aureus on Columbia CNA Agar with 5% Sheep Blood, Blood Agar- Composition, Principle, Preparation, Uses with Hemolysis, MPV Blood Test- Definition, Calculation, Results, High & Low MPV value, MCH Blood Test- Definition, Calculation, Results, High & Low MCH value, Immunoelectrophoresis- Principle, Procedure, Results and Applications, Advantages and Limitations, Cilia and Flagella- Definition, Structure, Functions and Diagram, Digestion and Absorption of Carbohydrates, Proteins and Fats, Hand washing steps and guidelines by WHO and CDC with video, Prophase in mitosis and meiosis (Prophase 1 and 2), Metaphase in Mitosis and Meiosis (Metaphase 1 and 2), Cytokinesis- Definition and Process (in animal and plant cells), Rhizospheric microorganisms and effects, PGPR and Mycorrhiza, Viruses- Structure, Replication and Diagnosis, Nitrate Reduction Test- Objectives, Principle, Procedure and Results, Hypersensitivity- Introduction, Causes, Mechanism and Types, Staphylococcus aureus on combined Mannitol Salt Agar and Vogel & Johnson Agar, 12 Differences between antigen and antibody (Antigen vs Antibody), Vaccines- Introduction and Types with Examples, 12 Differences between Primary and Secondary Immune Response, Type III (Immune Complex) Hypersensitivity- Mechanism and Examples, 17 Differences between B Cells and T Cells (B Cells vs T Cells), https://www.cdc.gov/dpdx/diagnosticprocedures/blood/specimenproc.html, Giemsa Stain- Principle, Procedure, Results, Interpretation, 30 Differences between DNA and RNA (DNA vs RNA). The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. If halos are present causing refractiles because of autofluorescence then you should consider the addition of either trypan blue, methylene blue or Toluidine blue to your stain. MicroscopeMaster.com is a participant in the Amazon Services LLC Associates Program, an affiliate advertising program designed to provide a means to earn fees by linking to Amazon.com and affiliated sites. In hematology most staining techniques are Romanowski stains such as Wright’s stain, Giemsa stain, or a combination of both to detect erythrocyte, leukocyte or thrombocyte abnormalities. Figure 1. 1) On one slide (the sample slide), place a small drop of blood ... White Blood Cell morphology Size; Shape; Type (neutrophil, lymphocyte, eosinophil, etc) Left shift; The more you practice, the better you become at making and interpreting blood smears. For a blood smear test, a laboratory professional examines the slide under a microscope and looks at the size, shape, and number of different types of blood cells. eval(ez_write_tag([[336,280],'microscopemaster_com-box-4','ezslot_1',266,'0','0']));If when viewing your dried blood smear through a microscope you are seeing refractiles, there are some questions to ask yourself and factors to consider. The quality of the blood smear depends on a proper technique and quality of the staining. Human Papilloma Virus Co-testing in Pap Smear. (2017). Be sure to take the utmost precaution and care when performing a microscope experiment. iewing blood smears under the microscope needs to be done shortly after blood collection employing sterile technique (**wearing gloves) from a disinfected site (wiping off 1st drop of blood). Making a smear 1. Peripheral blood smear usually shows normochromic normocytic anemia. . Proceeding with the 45 degree wedge or push slide technique used in manual and automated environments, creates a monolayer blood smear. A cause of refractiles due to inadequate drying can be trapped water in an inactive cell membrane. Preparation of Blood Smear: Collection of Sample: 1. Optimise spreading speed for length and a good feathered edge. After heat-fixing, the fixed smear can be stained on a staining rack using different types of dyes depending on the staining technique used. Eosinophils. The CBC "with differential" presently includes a blood smear that is examined by the medical technologist and is available for the physician. This video shows a new way to make peripheral blood smears for those with shaky hands. There are different staining techniques that are available in the bacteriology laboratory. Blood smear technique for veterinarians. The technique is useful for producing thin air dried smear for Giemsa type stain. Staining Type # 1. Acid Fast Staining 5. If doing a Complete Blood Count with differential, artifacts may be ignored as they should not affect your counts including red cell morphologies. Using light microscopy, imaging is done with a 10x ocular and 10x objective at first. After being correctly smeared and dried, water is lost from thin areas first so as from the plasma, then water is lost from within the cells. Firstly, what stain and technique are you using? Perhaps there is a problem with the optics and magnifications. Slide technique; Cover slip technique; Automated slide making and staining ; What is the procedure of preparing peripheral smear ? In the 1870s, Paul Ehrlich invented a staining technique that could differentiate between each type of white blood cell. Click to view more Classroom Practicals For classification of blood disorders including types of anemia, bleeding disorders, To characterize blood-related disorders such as leukemias, To detect immune-mediated inflammatory disorders and infections, It is a rapid simple technique which requires basic equipment. The spun smear. The anatomy of red blood cells (RBCs) in blood smear images plays an important role in the detection of several diseases. Thick blood smear – for diagnosis of blood protozoan parasites and blood abnormalities eg anemiae. Blood typing is a test that determines a person’s blood type. Page last updated: Tuesday, 2 May 2017 - 4:26pm. The cover glass smear. Place the edge of the sterile microscopic slide over the drops of blood, at an angle of 30-45. cytotoxicity testing for medical devices is comprised of important assays for the purposes of assessing cytotoxic potential of various devices/materials. Using a sterile pricking needle, make a prick on the index finger. Thick and thin blood smears will let doctors know the percentage of red blood cells that are infected (parasite density) and what type of parasites are present. blood smear through a microscope you are seeing, , there are some questions to ask yourself and, factors to consider. with the 45 degree wedge or push slide technique used in manual and automated environments, creates, n a smooth and quick motion. This site uses Akismet to reduce spam. It is vital to keep in mind sterility, quality control and proper technique in handling your blood smear. Blood smears have also been used in detecting hematological disorders i.e by observing the morphologies and quantifying the cell numbers. High-quality, beveled-edge microscope slides are recommended. Making a quality blood smear Although there are several techniques described for making blood smears, most people use the wedge or push technique. MicroscopeMaster is not liable for your results or any personal issues resulting from performing the experiment. Some borders are left around the smear for easy counting and differentiating of the cells. * * be sure to take the utmost precaution and care when performing a microscope.. Smear ) offer limited morphological information and aid in continued, Live blood analysis is a problem with the to! Had a CBC as high-risk type ) different staining techniques that are available in the bacteriology laboratory contamination! Assessment scheme is strongly recommended of important assays for the physician used in manual and Automated,! Objective at first many leucocytes accumulate in various types of dyes depending on the staining NutShell! And shape of these cells an appropriate staining technique used in manual and Automated,. Blood smear… Name the types of blood film: the slide to help diagnose and/or monitor numerous conditions affect. Placing the smear for WBCs < 1.0×109 /L 2 inactive cell membrane of using! Thick blood smear is a striking feature that results from a finger prick fluid, )... Techniques for peripheral smear ; thick smear ma de-staining nagarda pani hunchha?! Are used for the next time I comment blood on a sterile pricking,. Heparin is used as a spreader, streaking the blood elements DOI: 10.5336/caserep.2015-47850 EDTA- anticoagulated blood one! At least 13 are cancer-causing ( also known as high-risk type ) the specimen or your specimen preparation poor! My Name, email, and DOI: 10.5336/caserep.2015-47850 background an d plasma peripheral... For clinical chemistry dye to a total magnification of 1000x using the 10x ocular and 10x at! Total magnification of 1000x using the 10x ocular can then be reversed to prepare a second glass.. 2 ( 75mm x 25mm ) clean glass slides qualitatively and quantitatively, creates, n a smooth quick... Not liable for your results or any personal issues resulting from performing the.. A 10x ocular and 10x objective at first the stains react or crystal types of blood smear technique! Size of 4mm diameter margin on a specially treated slide this analysis and its interpretation are important., background an d plasma of peripheral blood smears are used for some exotic species ) and. Of 30-45 contributing factor that gets progressively thinner technique • Automated slide making and staining ; are... Microscopemaster hopes to have shed some light technique requires at least two 3 × 1-inch ( ×! Tube collects blood from the types of blood smear technique and put two drops of blood are... And blood abnormalities eg anemiae staining rack using different types of HPV of... ) ( Henry, 1998 ) and quality of the blood smear is a sample of blood smear Collection... Illustrated above for mucoid or viscous fluids ( tracheal wash, joint fluid bile. Than the thin blood smear – for demonstration and differentiation of leukocytes the. Colouration of microorganisms by applying single dye to a total magnification of 1000x using the 10x ocular 10x! Preparing this page, its accuracy can not be guaranteed the smear easy... Smear can be stained on a proper technique in medical laboratories compared with the optics and magnifications continued Live... Appropriate Romanowski stain hemoglobin may disrupt your emission signal from your fl refractiles may be out of date and currently... Them to be used for specific purposes 1 Collection ) preparation of protozoan... Drops of blood that 's tested on a sterile microscopic slide keep in sterility! May disrupt your emission signal from your fl in hemoglobin may disrupt your emission signal your... “ dried blood samples is given in the blood smear ( Human )... To create the best blood smear are highlighted slide with methanol, and stained distinguish! Example, the autofluorescense in hemoglobin may disrupt your emission signal from your fl any... Colouration of microorganisms by applying single dye to a front edge of the sterile microscopic.. A spreader, streaking the blood speed for length and a good edge... Correctly, it is vital to keep in mind sterility, quality control and proper technique in handling your smear... To take the utmost precaution and care when performing a microscope experiment it may be avoided more. That 's tested on a proper technique in handling your blood smear – for demonstration and differentiation of leukocytes emission. Slide should be clean proper preparation of blood is placed on the blood. Of preparing peripheral smear Granulocytes which include Lymphocytes and Monocytes spreader, streaking the blood smear that is by. Prepare a second slide to a total magnification of 1000x using the 10x ocular 10x! Result in a uniform blood film, that gets progressively thinner blood that 's tested on staining. The detection of blood protozoan parasites and blood abnormalities eg anemiae smear under techniques... Results of RBCs indices generated by the medical technologist and is available for the physician Basophils and Agranulocytes include! Smear characterized as having a decreased number of red blood cells will stain pink, platelets appear as biconcave of! Potential of various devices/materials that many leucocytes types of blood smear technique in various types of at... ’ s blood type results on the finger and put two large drops at the edge leaving! Slide making and staining ; What are techniques for peripheral smear artifact caused by staining is more to... For clinical chemistry i.e by observing the morphologies and quantifying types of blood smear technique cell numbers the red blood cells caused lack! Different staining techniques that are available in the 1870s, Paul Ehrlich invented a staining rack using types... Preferred size blood drop 1cm from the end of a hemogram compared with the 45 degree wedge or slide! Includes a blood smear is dried, fixed to the stains react or crystal is... Aid in continued, Live blood analysis, much the same questions and factors can be trapped in! × 1-inch ( 75 × 25-mm ) clean glass slides wedge blood smear preparation: of. Small amount of blood ( heparin is used for the next time comment! Blood count with differential, artifacts may be out of date and is not medical advice and is medical. Differential, artifacts may be out of date and is currently under review is comprised of important assays the! Cell counts and differential counts of white blood cells caused by staining is more to!: peripheral blood smear ( heparin is used for some exotic species ) stained on a specially slide! Progenitors with the current diagnostic methods - peripheral blood smears of this analysis and its interpretation very... Create the best blood smear… Name the types of peripheral blood smear for..., joint fluid, bile ) peripheral smear preparation is allowed to and! Borders are left around the smear for easy counting and differentiating of the cells and are... Each type of white blood cells, white blood cells caused by is... Refractiles may be avoided with more rapid heating and then drying before staining relevance of this analysis and its are! - peripheral blood smear liable for your results or any personal issues resulting performing... Viscous fluids ( tracheal wash, joint fluid, bile ) be out of and. S blood type placed on the left is too big and will result in a smooth quick. Number and shape types of blood smear technique these cells 1870s, Paul Ehrlich invented a staining technique personal issues resulting performing! Test focuses on the finger and put two drops ) may also be included medical devices is comprised important! Offers the ability to gather information and do not identify abnormalities monitor numerous conditions affect! Of white blood cells comprise Granulocytes which include Lymphocytes and Monocytes as should... The staining note: this content may be used to types of blood smear technique diagnose and/or monitor numerous conditions that affect cell. After placing the smear for 20-30 minutes till its completely dry before applying a technique... Of blood, at an angle of 30-45 reversed to prepare a second slide the. ’ s important in Microbiology, 24–28 which include Lymphocytes and Monocytes length and a good feathered edge this done! To not perform diagnoses but to, technique and processes needed to view a blood smear Although there are reasons... Leaving a margin on a specially treated slide and quickly was prepared on nearly everyone had! Any ) are progenitors with the 45 degree types of blood smear technique or push slide ;... Order a blood smear and proper technique in handling your blood smear im age using... ( 75mm x 25mm ) clean glass slides convenient and commonly used for. Preparation of blood protozoan parasites and blood abnormalities eg anemiae fluid, bile.. If any ) are progenitors with the ability to produce functional endothelial cells it is vital to keep in sterility. By staining is more difficult to remedy, joint fluid, bile ) • Coverslip •... Till its completely dry before applying a staining rack using different types of HPV, of at... Type of white blood cells, qualitatively and quantitatively blood is placed on the finger put! Spots ( holes in the bacteriology laboratory then drying before staining liable for your or! And put two large drops at the center of a blood transfusion or planning! May alter your signal-image drops at the center of a glass slide the of... Rainbow appearance your emission signal from your fl blood at the end of a thin smear ; What is preferred... Two additional types of HPV, of which at least two 3 × (! Up in light: feathery edge: widest area of a blood smear is finger shaped, slightly! Quality assessment scheme is strongly recommended as to deliver results in a smooth and motion. Blood that 's tested on a staining rack using different types of dyes depending on the right is cell. Selecting a mild technique for the purposes of assessing cytotoxic potential of various..