2018 May 29;11(1):317. doi: 10.1186/s13071-018-2902-9. We developed a SYBR Green real-time PCR restriction fragment length polymorphism assay (cytb-qPCR) targeting the cytochrome b gene of the four major human Plasmodium species (P. Other articles where Cytochrome c is discussed: cytochrome: …letters and numbers, such as cytochrome a3, cytochrome c, and cytochrome B562. Fast-evolving regions such as the "D-loop" might be used for studying closely related species while slowly-evolving regions such as cytochrome b (CYTB) and cytochrome oxidase I (COX1) might be used for distantly related species. Species identification using the cytochrome b gene of commercial turtle shells. The mitochondrial cytochrome b gene has been used in phylogenetic as well as in forensic investigations [10–12] and has been shown in a variety of studies to be a very useful DNA-region for species determination [13–18] However, before applicable for routine analysis, species-specific diagnostic polymorphisms or mutations must be determined. cytochrome b gene variability. Viral metagenomics of six bat species in close contact with humans in southern China. Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China. Would you like email updates of new search results? The vertebrate prepronociceptin gene (PNOC) was also tested in this field. He WQ, Xiong YQ, Ge J, Chen YX, Chen XJ, Zhong XS, Ou ZJ, Gao YH, Cheng MJ, Mo Y, Wen YQ, Qiu M, Huo ST, Chen SW, Zheng XY, He H, Li YZ, You FF, Zhang MY, Chen Q. BMC Vet Res. DNA typing in wildlife crime: recent developments in species identification. Epub 2008 Nov 28. NIH This chapter details a robust DNA technique using part of the cytochrome b gene on the mitochondrial genome that will work on poor quality samples such as powdered horn or ivory products including statues and carvings. Recent estimates of the number of species range from 10 to 100 million (Wilson 1998). Epub 2017 Oct 5. Please enable it to take advantage of the complete set of features! in R. norvegicus has no similarity with the cytochrome b gene from six other species, so it can be used as molecular markers to detect the presence of rat meat contamination in the processed of meat products. 2012;830:321-9. doi: 10.1007/978-1-61779-461-2_22. Amplification of part of the cytochrome b gene using universal primers produces a fragment of approx 486 bp in size. Direct sequencing of the PCR products allows comparison of the DNA sequence at this locus to those already described on the EMBL DNA database. The results of these analyses parallel and reinforce some of the principal trends in genetic distance estimates derived from … PCR-based screening and phylogenetic analysis of rat pegivirus (RPgV) carried by rodents in China. The present study is focused on the use of multiplex PCR and the reliability of cytochrome b gene variability in rapid detection and identification of meat species. | 2010 Sep;6(3):195-206. doi: 10.1007/s12024-010-9168-7. Forensic Sci Med Pathol. Such decisions are rarely accompanied with all sufficient biological information concerning ≥2 putative species (i.e., sympa… Would you like email updates of new search results? Many large mammalian species are on the verge of extinction in part because of the trade in their skin, bone, horn, or body parts for supposed medicinal purposes. The cytochrome b (cyt b) gene is one of the most commonly used genetic loci, applied in both taxonomy and forensic science, for the purpose of species identification. To date, studies on host preferences and blood meal identification have been conducted for Culicoides species using molecular-based methods such as PCR techniques to amplify only a fragment from universal vertebrate mitochondrial genes such as cytochrome c oxidase subunit I or cytochrome b (Cyt b). The use of molecular markers can enhance species identification, and acquires particular relevance in capture-release studies. Cytochrome c is highly water-soluble, unlike other cytochromes, and is an essential component of the electron transport chain, where it carries one electron. 2018 Jan;32:33-39. doi: 10.1016/j.fsigen.2017.10.003. In this study, we discuss the extent of the use of the mitochondrial cytochrome b gene for species identification, comparing external morphology identification with a molecular phylogenetic classification based on this marker, under the light of current bat systematics.